Inventaire d'enquêtes Demostaf

Information sur la citation

Type Revue - Tropical Medicine & International Health: TM & IH
Titre Antigen persistence of rapid diagnostic tests in pregnant women in Nanoro, Burkina Faso, and the implications for the diagnosis of malaria in pregnancy.
Auteur(s)
Volume 17
Numéro 5
Publication (Jour/Mois/Année) 2012
Numéros de page 550-557
URL http://proxyweb.ined.fr/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=cmedm&AN=224​69496&lang=fr&site=eds-live&scope=site
Résumé
Objectives: To evaluate persistence of several Plasmodium antigens in pregnant women after treatment and compare diagnostics during treatment follow-up.; Methods: Thirty-two pregnant women (N = 32) with confirmed malaria infection by a histidine-rich protein 2 (HRP2)-based rapid diagnostic test (RDT) and microscopy were followed for 28 days after artemisinin-based combination therapy (ACT). A Plasmodium lactate dehydrogenase (pLDH)-based RDT and two ELISAs based on the detection of dihydrofolate reductase-thymidylate synthase (DHFR-TS) and haeme detoxification protein (HDP) were compared with each other and to RT-PCR at each visit.; Results: The mean visit number (95% confidence interval) on which the HRP2-based RDT was still positive after treatment was 3.4 (2.7-4.1) visits with some patients still positive at day 28. This is significantly later than the pLDH-based RDT [0.84 (0.55-1.1)], microscopy (median 1, range 1-3), DHFR-TS-ELISA [1.7 (1.1-2.3)] and RT-PCR (median 2, range 1-5) (P < 0.05), but not significantly later than HDP-ELISA [2.1 (1.6-2.7)]. Lower gravidity and higher parasite density at day 0 resulted in significantly longer positive results with most tests (P < 0.05).; Conclusions: HRP2 can persist up to 28 days after ACT treatment; therefore, this test is not suitable for treatment follow-up in pregnant women and can generate problems when using this test during intermittent preventive treatment (IPTp). DHFR-TS is less persistent than HRP2, making it a potentially interesting target for diagnosis.; © 2012 Blackwell Publishing Ltd.

Études utilisées

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Kattenberg, Johanna H, Christian M Tahita, Inge A J Versteeg, Halidou Tinto, Maminata Traoré-Coulibaly, Henk D F H Schallig, and Petra F Mens. "Antigen persistence of rapid diagnostic tests in pregnant women in Nanoro, Burkina Faso, and the implications for the diagnosis of malaria in pregnancy.." Tropical Medicine & International Health: TM & IH 17, no. 5 (2012): 550-557.
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